Ozone disinfects by directly oxidizing and destroying the microorganism’s cell wall, causing cellular components to leak outside the cell. This causes protoplasmic destruction of the cell, damaging constituents of the nucleic acids, and breaks the carbon–nitrogen bonds, which leads to depolymerization. During the process, ozone splits into oxygen and an ozone atom, which is lost during the reaction with the microorganism’s cell fluids, O3 -> O2 + (O).

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The ozonated water is produced on the vehicle and delivered directly into the plant or playing surface. The  system is a sealed unit there is no danger from Ozone gas generation (“Off Gas”). Due to the nature of the direct oxidation power of ozone, there is no mechanism for pathogens to create an immunity to ozone as with chemical disinfectants and biocides.